The vortex not only provides a different profile of mixing, but has the power to mix viscous fluids that would otherwise remain virtually unmixed (glycerol stocks come to mind). Vortex shakers are contra-indicated for some lab protocols (like in miniprep kits) …

Feb 4, 2016 · $\begingroup$ I want to point out about undergrad labs: People vortex SDS mixtures and small volumes, and advertently the pipettes can't get everything out of the tube reasonably sometimes due to bubbles. You could try to centrifuge it, but if there are still bubbles, trying to pipette 50µL of bubbles is annoying.

Oct 31, 2012 · It's a curiosity question. When I'm doing minipreps after pelleting the bacteria sometimes it's very easy to resuspend them in P1 (Qiagen kit), but sometimes they form a rubbery clump that is very hard to break up with a pipette and I have to vortex the tubes. It interests me because it adds more time to my lab work and I wish I could avoid it.

Vortex & lightly spin down on a centrifuge. Add the required amount of TAQ to master mix (insert in to the liquid) and mix using the pipette to draw some liquid up and down. Add master mixes to labeled PCR tubes in the required volumes as specified by the protocol.

Mar 28, 2016 · I am trying to do some DNA extraction for my professor, and I make a lysis buffer with proteinase K.I did not have the time to continue the procedure so the hair and the buffer have been sitting together at room temperature for about 12 days.

Jun 2, 2017 · $\begingroup$ we washed mouth twice with listerine to reduce bacteria count, then scraped cheek cells with moistened ear bud then smeared that on clean glass slide. then air drying the smear we fixated it with methanol, then after air drying we applied thin layer of giemsa stain and after 15 minutes drained and washed off extra stain. the cells were stained and we …

Apr 19, 2014 · The loading buffer you add to your samples for gel electrophoresis has a few different purposes, but the exact amount does not really matter.

I would suggest taking one or two protein samples and doing a dilution series (1:1, 1:5, 1:10, 1:20) in a large-ish volume (say 400 ul each, if you can spare it), vortex briefly to mix well, then measure triplicates of each dilution on your reader, along with appropriate blanks (buffer only).